Bacterial Transposons containing Markers for Fungal Gene Disruption
نویسندگان
چکیده
منابع مشابه
Bacterial transposons.
Bacterial plasmids have been known for more than twenty years and, during this period, one witnessed an extraordinary spread of those extra-chromosomal DNA elements. Resistance plasmids were first identified in Enterobacteriaceae but, later On. have been found in almost every pathogenic bacterial species, including more recently Hemophilus influenzae and Neisseria gonorrheae. Plasmids, however....
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DNA based transposon vectors offer a mechanism for non-viral gene delivery into mamma‐ lian and human cells. These vectors work via a cut-and-paste mechanim whereby transpo‐ son DNA containing a transgene(s) of interest is integrated into chromosomal DNA by a transposase enzyme. The first DNA based transposon system which worked efficienty in human cells was sleeping beauty. This was followed a...
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With the process of evolution, some genes will change their relative positions in gene sequence. These “jumping genes” are called transposons. Through some intuitive rules, we give a criterion to determine transposons among gene sequences of different individuals of the same species. Then we turn this problem into graph theory and give algorithms for different situations with acceptable time co...
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The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for analyzing gene function. Here we describe the addition of ∼7600 new strains, which were selected from >140,...
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A simple, inexpensive method for disrupting dormant fungal spores is described.
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1997
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1276